In view of underwater acoustic complex signal and expensive data acquisition card, based on sound card acquisition and virtual instrument technology, a multi-function underwater acoustic signal generator and data acquisition display control system generator method are proposed. And the sound card and NI-DAQ devices are used to realize multi-function underwater acoustic signal control, transmission, display and audio signal acquisition, display based on the platform of LabVIEW. The system not only could be used as a virtual signal source for generating the desired signal used by underwater acoustic transmitter, but also the sound card could be used to acquire and measure audio analog signal, then display the signal in real time. The emission signal generated by the system is acted on an underwater acoustic power amplifier and practical applications are combined with for the purpose of laboratory validation. Results indicate that the system is able to effectively generate and control the actual signal needed. The module design for both control output and gathering input has high degree of integration, versatility; the hardware cost is reduced. The system possesses the advantage over friendly interface, low cost, and simple operation for spreading.