Currently, arbuscular mycorrhizal fungi technology becomes one of the hotspots in the research on land microbial remediation for mining subsidence. Taking amorpha fruiticosa planting in the mining subsidence of Shendong mining area as a study object, AMF vaccinated on amorpha fruiticosa after 16 months, 25 months, and 28 months, respectively are used to analyze its effect on the growth of plant and on rhizosphere soil physical properties. And then the correlation between hyphae density and effective phosphorus of rhizosphere is analyzed. By using regression analysis, it is indicated that in different monitoring time, for example, hyphae density and effective phosphorus in September had better linear fitting than that in June, which is closely associated with development time of mycorrhizal. With the time passing by, the correlation of vaccination between hyphae density and effective phosphorus content is gradually decreased; however, the positive correlation of un-vaccinated is gradually emerged. The results of mycorrhizal bioremediation on mining subsidence in Shendong mining area prove that vaccination not only could effectively promote growth and development of amorpha fruiticosa in macroscopic, but also could improve the growth matrix conditions in microscopic.