LIU Haiyan;WANG Xi;ZHONG Gansheng;LI Yiwen;LIU Jia;OU Lina;LIU Yunxiang;GAO Yuan;CHEN Shaohong
By the detection of the liver function and renal function of mice in acute toxicity test that were given Gansuibanxia decoction that adds or subtracts Gansui and Gancao anti-drug combination, we expect that these results provide evidence for acute toxicity experiments. The male Kunming mice of 18-20 g were randomly grouped, including the blank group, the group of Gansuibanxia decoction, the group of Gansuibanxia decoction with Zhigancao removed, the group of Gansuibanxia decoction with Cugansui removed, the group of Gansuibanxia decoction with Zhigancao and Cugansui removed, the group of Zhigancao, the group of Cugansui,the group of compatibility of Zhigancao and Cugansui. Each administration group was divided into five dose groups by diluting to a ratio of 1:0.85. Blank group was given distilled water, the rest groups were given the drugs. The method of administration was maintained for 28 days, then the eyes of the mice that did not die were removed and centrifugated, to detect serum alanine aminotransferase(ALT), aspartate aminotransferase(AST), alkaline phosphatase(ALP), creatinine(CRE), blood urea nitrogen(BUN) levels. The AST results show that, compared with the blank group, the serum AST activity of the group of Gansuibanxia decoction 1, 2 and 3, the group of Gansuibanxia decoction with Cugansui removed 2, 3 and 5, the group of Gansuibanxia decoction with Zhigancao removed and Cugansui 4, the group of Zhigancao 2, 3, 4 and 5, the group of Cugansui 1, 3, 4 and 5, the group of compatibility of Zhigancao and Cugansui 2, 3 and 5 were higher than the blank group and had statistically difference. The ALT results show that compared with the blank group, the serum ALT activity of the group of Cugansui 4 was higher than the blank group(P<0.01). The serum ALT activity of the group of Gansuibanxia decoction with Zhigancao removed 4 was lower than blank group(P<0.05). The serum ALT activity of the group of Zhigancao 2, the group of Cugansui 1, 3 and 5, the group of compatibility of Zhigancao and Cugansui 1, 2, 3 and 5 were higher than the blank group(P<0.05). The blood ALT activity of the rest groups in comparison with the blank group does not have a statistically significant difference(P >0.05). The ALP results show that the serum ALP activity of all the groups does not have a statistically significant difference compared with the blank group. The BUN results show that compared with the blank group, the serum BUN activity of the group of Gansuibanxia decoction 5, the group of Gansuibanxia decoction with Zhigancao removed 2 and 5, the group of Gansuibanxia decoction with Cugansui removed 2 and 3, the group of Gansuibanxia decoction with Zhigancao and Cugansui removed 1 and 2 were lower than the blank group(P<0.01). The serum BUN activity of the group of Gansuibanxia decoction with Cugansui removed 4, the group of Gansuibanxia decoction with Zhigancao and Cugansui removed 3 and 5, the group of Cugansui 2 were lower than the blank group(P<0.05). BUN decreased in the blood of mice that did not die in the experiment of LD50(n), and it is a kidney injuryhas yet to be further experimentally verified. The CRE results show that all of the blood CRE content in comparison with the blank group did not have a statistically significant difference(P >0.05). It can be seen that in the experiment of LD50(n), each group of drugs on the liver and kidney of mice mainly has a certain degree of damage to the liver, and the liver injury of anti-drug combination of the Gansui and Gancao application in the compound compared with two separate application is decreased. In the LD50(n) experiments, the compound containing anti-drug combination of Gansui and Gancao in a certain dose and time of administration showed a certain liver damage of not died mice may come from the anti-drug combination of Gansui and Gancao.
