28 June 2013, Volume 31 Issue 18

    

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  Effect of Novel TiO₂ Nanotube Dialysis and Traditional Dialysis Films on Cells Growth State and Their Comparison

  ZHU Wen;LI Jiwei;LIU Jianfeng;TAN Cong;CHONG Baohe;YANG Xuebing

  . 2013, 31(18): 15-21. https://doi.org/10.3981/j.issn.1000-7857.2013.18.001

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  Novel high intensity TiO₂ nanotube array film was fabricated via electrochemical anodization, and then open-ended TiO₂ nanotubes array membrane was obtained by HF gas etching the bottom of nanotubes. HK-2 cells and HUVEC cells were cultivated on the open-ended TiO₂ nanotube array surface and polymer dialysis film surface, and biomembrane materials which have physiological function are successfully achieved. Cell viability of TiO₂ nanotube array, Polyether Sulfone (PES), mixed cellulose ester, and regenerated cellulose was investigated by using MTT method. Cell morphology on these membranes was examined by using fluorescence microscope and Filed Emission Scanning Electron Microscope (FESEM). The results indicate that TiO₂ nanotube array film is of the most benefit to cell adhesion and cell proliferation, and the cell viability is the highest, and the second place belongs to the PES membrane, the worst case is regenerated cellulose that is unsuitable for cell proliferation and cell adhesion. Fluorescence microscope shows that TiO₂ nanotube array membrane could promote the cell adhesion and cell proliferation batter than polymeric membrane does. It demonstrates that TiO₂ nanotube array film has excellent biocompatibility, overcoming the deficiency of traditional dialysis materials, improving the cell adhesive; and the film is the ideal candidate materials for biological dialysis.
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  Impact of the Height of Gravity Center to Hydrodynamic Performance of Floating Structure

  CHEN Zhan;MA Yong;ZHANG Liang;WU Minghui

  . 2013, 31(18): 22-26. https://doi.org/10.3981/j.issn.1000-7857.2013.18.002

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  In general, the external structures are the main factors influencing the hydrodynamic performance in the computation simulation, however, the impact of gravity center on the hydrodynamic calculation is ignored. In order to study the effect of gravity center change on the structure hydrodynamic response, a 5MW floating wind power turbine system is taken as the research object. By means of numerical simulation method, the relationship between the height change of gravity center and float spacing, bar form as well as heave plate, respectively is studied. The results show that when the float distance changing, the effect of gravity center height on hydrodynamic performance is mainly reflected in the cross, longitudinal and transverse, longitudinal swing, and when the basic form of larger float distance reaches at the peak response, the gravity center is even lower; the change of diagonal brace is consistent with the rule of motion amplitude along with gravity center changing, and each response amplitude after peak response in terms of numerical value is basically the same; the heave plate adding is able to effectively reduce the pitching and heaving amplitude; for the three floating semi-submersible platform, the best central location is located at the underwater of 3-8m.
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  Implementation of Both Additional Information Extraction and Image Display from MR Image

  WAN Suiren;GU Cuiyan;SUN Yu

  . 2013, 31(18): 27-31. https://doi.org/10.3981/j.issn.1000-7857.2013.18.003

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  Additional figure information in MR images is able to provide guidance for Magnetic Resonance Spectrum (MRS), helping located lesions, and it has certain clinical application significance. While several free Digital Imaging and Communication in Medicine(DICOM) Viewers are able to display DICOM image files and key information, however it still has some shortages in the applications to the area of scientific research. First at all, their major basic function is just to display images. In some extent, they could only give the assistance to the research of magnetic resonance data. And then they are unable to completely display some special kinds of DICOM images, such as Siemens/GE magnetic resonance imaging. The location information of MRI images would be often lost in it. Above all, a program named dicomreader that displays DICOM image properly is developed by using Java and the open source toolkit of dcm4che. The process for extracting overlay pixels and displaying additional information on the DICOM images is also proposed. Finally, the experimental results show that dicomreader is able to implement the conversion from DICOM to image quickly and display the additional information precisely. Meanwhile, the additional information extracted could be used by research personnel for locating the pathological change of organism, such as brain tumor, three-dimensional reconstruction of brain organism, the partition of MRI images, and clinic diagnosis based on the information of wave spectrum.
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  Complex Dynamical Behavior of Rigid-flexible Coupling Spring Pendulum

  YU Hongjie;ZHANG Jingshu;HONG Jiazhen

  . 2013, 31(18): 32-38. https://doi.org/10.3981/j.issn.1000-7857.2013.18.004

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  Aiming at the time-varying, strong nonlinear rigid-flexible coupling multi-body systems with both variables of fast and slow speeds, qualitative analysis is carried out and complex dynamical behaviors are numerically simulated by using a spring pendulum model. A dimensionless dynamical equation of the two-time scale variable rigid-flexible coupling spring pendulum system is established. A more appropriate numerical method for solving stiff problems is compared and selected from the perspective of energy conservation. The frequency ratio between different time scale variables and the initial value of swing angle are taking as the control parameters; on the condition of greater frequency ratio and the large range of initial swing angle, the complex dynamical behavior of wide range swing coupling with small amplitude oscillation is analyzed by means of the numerical simulation of spring pendulum system. The parameter domain for the different dynamical behavior corresponding to different time scale variables is given within a certain range. Results indicate that two-time scale variable system has a complex dynamical behavior including chaos with the change of the frequency ratio between the different time scale variables and the change of initial conditions. Especially, the initial value of large swing angle is easier to cause the chaotic behavior of different time scale variables. A foundation for further qualitative analysis and numerical simulation studies on the dynamical behavior of rigid-flexible coupling multi-body systems is laid down and references and evidence are provided.
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  Effects of Surface Cracks on Microorganisms Quantity and Enzyme Activities in the Rhizosphere of Artemisia ordosica and Its Dynamic Evolution Characteristics

  DU Tao;BI Yinli

  . 2013, 31(18): 39-43. https://doi.org/10.3981/j.issn.1000-7857.2013.18.005

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  In contrast with those Artemisia ordosica which does not been passed through by the cracks caused by underground coal mining in Shendong mining area, Bulianta coal mine, the dynamic evolution characteristics of microorganisms quantity and enzyme activities of soil in the rhizosphere of A. ordosica passed through by the cracks are analyzed by using statistical analytical method. The results show that after the surface cracks appeared, the number of bacteria and actinomyces in the rhizosphere of A. ordosica is reduced, the number of fungi and the activity of enzyme are increased, the amounts of water content and electric conductivity are decreased, therefore the interferences of surface cracks on the biological activity in the rhizosphere of A. ordosica are quite obvious. After more than a year, with surface cracks gradually close, the content of soil water gradually returned to the normal, the effects of surface cracks on phosphatase activity, sucrase activity, and electric conductivity are gradually weakened, while the effect of surface cracks on the number of microorganisms does not change obviously. Nevertheless, the biological activity in the rhizosphere of A. ordosica possesses certain self-repair ability; however the complete recovery of biological activity might take a longer time.
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  Liquid Carbon Dioxide Fire Extinguishing Equipments and Their Engendering Applications

  ZHANG Chunhua;WANG Jiren;ZHANG Ziming;ZHANG Yadong

  . 2013, 31(18): 44-48. https://doi.org/10.3981/j.issn.1000-7857.2013.18.006

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  Coal mine fire, as one of the main disasters in the coal mine, not only could burn down coal resource and electromechanical equipment, but also might induce methane or dust explosion. The equipments for Liquid Carbon Dioxide (CO₂(l)) storage and transportation, the ground vaporous CO₂(l) fire extinguishing system, and the underground CO₂(l) direct fire extinguishing system are independently researched and developed. The self—pressurization system of the storage tank is able to make the CO₂ keep a liquid state without icing. For the ground vaporous CO₂(l) fire extinguishing system, the CO₂(l) is changed into gaseous CO₂ through the electrothermal vaporization and air temperature vaporization. And then the vaporous gaseous CO₂ is piped into a pressure stabilizing tank at a stable value of 1.5MPa. The gaseous CO₂ from the pressure stabilizing tank is transported at a pressure of 0.5MPa down to the coal mine in order to prevent or to extinguish the coal mine fire. The CO₂(l) could be directly transported by the fire extinguishing train consisting of a number of single storage tanks near to the underground high temperature zone or the fire zone, which is able to rapidly cool the high temperature zone or extinguish the fire. The CO₂(l) fire xtinguishing equipments are used to extinguish fire in the 122 fully mechanized workface of Zhaojin Coal Mine. The application results show that the scientific equipments are able to rapidly and effectively reduce the temperature, the concentration of oxygen and coal spontaneous combustion index gas in the fire zone, eliminate the hazards of target fire.
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  Energy Consumption Analysis on Fiber Preparation Unit of Fiberboard

  GE Bing;HUA Jun;JIA Na;SONG Jie

  . 2013, 31(18): 49-52. https://doi.org/10.3981/j.issn.1000-7857.2013.18.007

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  Statistical experiment method is applied to conduct energy consumption analysis on the fiber preparation unit of domestic typical fiberboard production manufacturer, the energy consumption composition for producing unit quality fiber, the share of energy consumption, and the main factors affecting fibers unit energy consumption are obtained. Through the test data of actual production, for the each process of fiber preparation unit, the consumption of electric energy and thermal energy in terms of standard coal is quantitatively analyzed and calculated. Based on the analysis and calculation, results indicate that the total energy consumption for 1t fiber is 162.434kg(ce); the ratio of electric energy consumption to heat energy consumption is about 1:4.49. Among the processes, dry and hot mill are the process of main energy consumption, accounted for 65.199% and 33.681% of the total energy consumption, respectively; the consumption proportion of electric energy and heat consumption is 1:17.4 and 1:1.75, respectively; and the research provide the theoretical basis for taking further effective stapes saving energy and reducing consumption.
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  MCNP and DORT Couptation Method

  YANG Defeng;XUE Na;Cheng Heping;MAO Yawei

  . 2013, 31(18): 53-56. https://doi.org/10.3981/j.issn.1000-7857.2013.18.008

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  Shielding is a common measure for the radiation protection. In the design process, radiation shielding calculation using computer codes is employed to achieve the proper shielding plan. MCNP and DORT are common programs used for computing radiation shielding. Based on different principles and algorithms, each of these programs has its own defect at certain areas. Achieving MCNP and DORT coupling is taken aim at, by studying the functions and features of MCNP and DORT, and the interface code is programmed. The method for converting data between MCNP and DORT is realized, Coupling between MCNP and DORT is achieved. So that the application scope of these two programs is able to be enlarged. After the validation, the conclusion is drawn that the interface code is able to convert the input and output files of MCNP and DORT successfully. Both accuracy and efficiency for coupling calculation are acceptable, having quite good management ability for all kinds of problems. The coupling method has been applied to technical problems and would be further validated by the feedback data.
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  Simulation of Coal Induce-crack Characteristics on the Conditions of Pulsatile and Constant Pressure Water Injections

  ZHU Hongqing;SHEN Jian;ZHANG Minbo;FENG Shiliang;XU Jiyuan

  . 2013, 31(18): 57-62. https://doi.org/10.3981/j.issn.1000-7857.2013.18.009

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  To solve the problem that fracturing effect is not perfect in the practical applications of coal constant pressure water injection, based on the application of FLAC3D computer simulation to the rock and soil mechanics, the crack initiation process of the coal on the conditions of pulsatile and constant pressure water injections is simulated, the induce-crack characteristics of the coal on the conditions of pulsatile and constant pressure water injections are analyzed. The results of numerical simulation show that after hydraulic fracturing, the fracturing radius of the strike direction in the model of pulsatile water injection is 3.3m, the fracturing radius of the vertical direction is 2.2m; the fracturing radius of the strike direction in the model of constant pressure water injection is 0.65m, the fracturing radius of the vertical direction is 0.96m. The coal needed more time to crack initiation on the condition of pulsatile water injection than that of constant pressure water injection, the ultimate compressive stress for the model of the pulsatile water injection is lower than that for the model of the constant pressure water injection, a conclusion that the pulsatile water injection is significantly better than the constant pressure water injection in the pressure relief of the coal-rock is drawn.
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  Oxidative Stress Reaction of the Model Combining Disease with Syndrome for Alzheimer's Disease Carrying Abnormal Savda Syndrome and the Intervenient Experiment of Prescription

  WUSHUER Palidan;UPUR Hamulati;KUERBAN Abudukadeer;AIZEZI Renaguli;DANG Ming;AIMAITI Nuermaimaiti

  . 2013, 31(18): 63-67. https://doi.org/10.3981/j.issn.1000-7857.2013.18.010

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  In order to study the antioxidant enzyme activity of hippocampal tissue in the rat model of Alzheimer's Disease (AD) carrying abnormal savda syndrome and observe the effect and mechanism of prescription on the oxidative stress, 36 male Wistar rats are randomly divided into six groups (six rats per group). A rat model combining disease with syndrome is established, three days latter, three groups are given Yihei (abnormal savda) granula for 15 days. The spectrophoto-metric method is employed to test the activity of SOD, GSH-Px, CAT, MAO and the content of MDA in the hippocampus of rat cerebrum. It is found that.the activity of SOD, GSH-Px, and CAT is obliviously decreased, the activity of MAO and the content of MDA is significantly increased in the rat hippocampus of the model group, compared with the normal group, the difference is significant (P<0.01). After the treatment, the group given high dose of Yihei granula demonstrates that the activity of SOD, CAT, GSH-Px (P<0.01) increase, and the activity of MAO, content of MDA are decreased in rat hippocampus (P<0.01). Compared with the model group, the difference is significant (P<0.01), However, compared with Donepezil group, there is insignificant difference between these two groups (P>0.05). The group given mild dose of Yihei granula demonstrates that the activity of SOD increases, compared with the model group, the difference is significant (P<0.01). The activity of GSH-Px, CAT is increase, the activity of MAO is decrease, compared with the model group, the difference is significant (P<0.05), the content of MDA does not improve obviously, compared with normal group, the difference is insignificant (P<0.01). The group given low dose of Yihei granula is compared with the model group, there is insignificant difference in the activity of SOD, CAT, MAO, and the content of MDA(P>0.05). Compared with normal group, the difference is significant (P<0.01). There is significant difference in the activity of GSH-Px compared with model group (P<0.05). Therefore, the rat model combining disease with syndrome does an injury to free radical, oxidative stress is excessive, and Yihei granular is able to improve the activity of antioxidant enzyme, reduce the content of MDA, and inhibit the incidents of AD by antioxidative effect. It might be one of the formula mechanisms for the AD treatment.
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  Effects of Different Dosage Phosphatic Fertilizer on Growth and Physiological Characteristics of Zoysiagrass (Zoysia japonica)

  CHEN Zhonglin;HE Yue;LI Yue;XU Sunan;ZHANG Lihong

  . 2013, 31(18): 68-72. https://doi.org/10.3981/j.issn.1000-7857.2013.18.011

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  Topdressing is an important link in the turfgrass management. The method of pot culture is used in order to investigate whether or not phosphatic fertilizer is necessary and the proper application rate of the fertilizer during the period of plant growth. The study is carried out to examine the effects of different dosages phosphatic fertilizer (0, 30kg/hm², and 60kg/hm² P₂O₅) on the growth and photosynthetic attributes of zoysiagrass (Zoysia japonica). The results show that treatments of 30g/hm² and 60kg/hm² P₂O₅ significantly increase the above and under ground biomass of zoysiagrass compared with controls. 30kg/hm² P₂O₅ treatment increases the contents of soluble protein, soluble sugar, and praline of zoysiagrass leafs, while 60kg/hm² P₂O₅ treatment decreases the content. Treatments of 30 kg/hm² and 60kg/hm² P₂O₅ enhance chlorophyll contents, net photosynthetic rate, stomatal conduction, transpiration rate, maximum photo quantum efficiency, and the electron transfer rate compared with controls, however decrease water use efficiency. During growth period, phosphatic fertilizer promotes the growth and development of zoysiagrass, improving the turfgrass quality of zoysiagrass. Every indicator of 30kg/hm² dosage phosphatic fertilizer is better than that of 60kg/hm² dosage phosphatic fertilizer.
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  Prospects in Edible Fungi Photobiology and LED Applications

  LIU Wenke;YANG Qichang

  . 2013, 31(18): 73-79. https://doi.org/10.3981/j.issn.1000-7857.2013.18.012

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  Edible fungi are the nutritive and healthy food, keeping consumer in good health and guiding national food security. China has become the biggest country for edible fungi production worldwide with many kinds of cultivation species. However, there are some problems for edible fungi production in China, including small scale, outdated technology, and development imbalance between regions. In the past decade, the market demand for edible fungi has increased annually. Therefore, in order to improve the yield and nutritional quality of edible fungi, factory production technology becomes an urgent task. Factory production of edible fungi is referred to cultivate edible fungi under controllable closed facilities all the year round. Since the nutrition patterns and environmental requirements for edible fungi are completely different comparing with the plants. Light is a necessary environmental factor that is needed in the formation of fruiting body primordium and the development of fruiting body. Various edible fungi pose different photobiological traits to light quality and light intensity. In fact, traditional electric sources are unable to meet diverse and precise light environment for the factory production of edible fungi. As a kind of semiconductor light source, Light-Emitting Diode (LED) has many advantages over traditional electric light sources in terms of light quality, light efficiency, and spectral energy distribution. Nowadays, LED becomes an ideal light source for the factory production of edible fungi due to the decline of LED price and enhancement of lighting emitting. The advances in the photobiology of edible fungi are summarized, and the application prospects of LED in the factory production of edible fungi are emphasized.