18 March 2012, Volume 30 Issue 8

    

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  Synergistic Anticancer Effect of Z18 Combined with S8 in vitro

  ZHOU Cuihong;WANG Xiaokui;ZHOU Jun;BI De;DENG Xiaoyan

  . 2012, 30(8): 20-24. https://doi.org/10.3981/j.issn.1000-7857.2012.08.001

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  To study the synergistic anticancer effect of Z18 combined with S8 in vitro against HeLa cell, we firstly determined the cell death rate using the trypan blue exclusion method and the necrotic cell death was identified by flow cytometry. After being confirmed the autophagy induction in Z18-treated cells, we next analyzed LC3-II conversion by the western blotting and used GFP-LC3 or mRFP-EGFP-LC3 expressing cells to check the autophagic activity and the autophagic flux after the combination treatment. Finally, we inhibited the autophagic activity or the autophagic flux by using ATG5 siRNA or CQ and determined the cell death rate after the combination treatment. It is shown that the pharmacologic inhibitor of Fatty Acid Synthase (FAS) S8 would promote the Z18-mediated cell death in HeLa cells (P<0.05) with the increase of the concentration of S8 and the cell would die mainly as a result of necrotic processes. However, when Z18 is combined with S8, S8 has no effect on the autophagic activity induced by Z18, such as LC3-II conversion. Inhibited autophagic activity has no effect on the cell death after the combination treatment. It is suggested that S8 does not change the autophagic activity but increase the cell death rate in HeLa cells. The enhancement of Z18 anti-tumor activity by S8 may be through another pathway of cell necrosis, and is not related to the autophagy pathway.
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  Serum Metabonomics Research on Shenxu-tanyu Syndrome Type 2 Diabetes Mellitus Based on NMR

  YOU Li;MAMTIMIN Batur;UPUR Halmurat

  . 2012, 30(8): 25-29. https://doi.org/10.3981/j.issn.1000-7857.2012.08.002

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  To explore the metabolic variation and pathogenesis of type 2 diabetes mellitus of Shenxu-tanyu syndrome in Traditional Chinese Medicine (TCM) by using Nuclear Magnetic Resonance (NMR). Serum samples from 33 cases of Shenxu-tanyu syndrome type 2 diabetes mellitus patients and 50 cases of healthy people are detected and described with 1H-NMR based metabonomics, and than the data is analyzed by Orthogonal Partial Least Squares-Discriminant Analysis (OPLS-DA). The results show that comparing with the healthy people, the various kinds of amino acids of Shenxu-tanyu syndrome type 2 diabetes mellitus patients are markedly lower, including isoleucine, leucine, valine, alanine, glutamate, histidine, tyrosine, phenylalanine, and 1-methylhistidine, the differences are statistically significant (P<0.05). In addition, lactate, citrate, creatine and creatinine are also markedly lower (P<0.05). However, the α-glucose and β-glucose are increased comparing with the health people (P<0.05). It is concluded that the metabolites of shenxu-tanyu syndrome type 2 diabetics are significantly different from healthy people, their tricarboxylic acid cycle and glycolysis are inhibited, protein is metabolic disorder, and branched chain amino acid is metabolic boost.
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  Sedimentary Facies of Large-scale Shallow Delta ——Taking the 4th Member of the Quantou Formation in Xinli Oilfield as an Example

  LI Junjun;WANG Zhizhang;ZHANG Zhihuan;LI Weihua;MA Fujun;WANG Ruyi

  . 2012, 30(8): 30-36. https://doi.org/10.3981/j.issn.1000-7857.2012.08.003

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  Based on the systematic study of the sedimentary characteristics, sedimentary, micro-facies, and sedimentary evolution of Fuyu reservoir, the following conclusions are obtained. During the deposition of the 4th member of the Quantou formation in southern Songliao basin, the basement sinks stably and slowly, the terrain is gentle and smooth slope. Under the background of lake basin expansion and shallow, river flees into the lake, forms a large shallow lake delta deposition, and divides into two sub-phases, namely, delta plain and delta front. The former delta facies include branch channel, nature levee, crevasse splay, and inter branch channel; the later delta facies include subaqueous distributary channel, subsea natural levee, sub-sea inter branch channel, mouth bar, and distal bar. The shallow delta sedimentary model forms as the result of strong hydrodynamic and the fast water invasion. The direction of the remainder oil is pointed out, and the adjustment for developing project with guiding significance is provided.
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  Analysis of Pressure Strain of Sandstone-type Gas Storage in North Banzhong Area

  SHI Lei;XIONG Wei;GAO Shusheng;WU Hongshuang

  . 2012, 30(8): 37-40. https://doi.org/10.3981/j.issn.1000-7857.2012.08.004

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  Underground Gas Storages (UGS) are widely used to store the surplus of produced natural gas during periods of low demand, and to relieve the pressure during periods of high demand. A depleted natural gas reservoir is generally the ideal choice for the UGS. The proper selection of a depleted gas reservoir is essential to the stability of the UGS in the long run. The storage ability is an important index for the performance of the gas storage. In order to analysis the stability of the storage ability of the UGS in BZ area, the development characteristics of the main reservoir are studied, with the pressure strain data of the reservoir analyzed and compared. The effect of the pressure strain on the porosity is evaluated by using mathematical formulas, and it is believed that the original porosity and the operation pressure are related with the effect of the pressure strain on the UGS. The case production history fitting of the gas storage has verified the analytical method and the mathematical chart board, which may offer an accurate and quantitative analysis of the storage ability in the UGS, and provide an analytical tool in the practical production management.
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  Predictive Model of Steel Plate Temperature in Heat Treatment Furnace Based on LS-SVM

  LI Jing;XIE Ting

  . 2012, 30(8): 41-44. https://doi.org/10.3981/j.issn.1000-7857.2012.08.005

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  According to the process characteristics of the medium thickness steel plate temperature control in the heat treatment furnace, a new steel temperature predictive model of heat treatment furnace based on the Least Squares Support Vector Machine (LS-SVM) of mixtures of kernels is established; the model also gives considerations to the some problems, such as the existence of local minima and the choice of the number of hidden units in traditional neural network. Based on the new model, the design steps are given. The LS-SVM model is able to fit the complex nonlinear functional relation between the input and output. Roll speed, steel plate length, width, thickness, upper and lower temperature of furnace, surface temperature of steel plate in previous moment are selected as inputs, surface temperature of steel plate in this moment is chosen as outputs. The very good forecast effect is obtained when on-site production process data are taken as the training samples to train the model, and then the data samples of model test are selected to simulate it. The model is used to calculate the steel plate surface temperature of the heat treatment furnace. The result indicates that the model is quite simple, and has the strong predictive ability and a broad prospect of applications.
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  High-order Staggered Finite-difference Numerical Simulation of While-drilling Seismic Wave Field

  DENG Rui;ZHANG Liang

  . 2012, 30(8): 45-50. https://doi.org/10.3981/j.issn.1000-7857.2012.08.006

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  The while-drilling Vertical Seismic Profile (VSP) is a new kind of geophysical prospecting techniques which uses the noise of drill vibration as its seismic source and receives signal by the wave detector deployed on the earth. The vibration of rock-drill is random and continuous, and the wave signal is weak. However, the noise in the well field is strong, and the waves of different times and seismic phases superpose and interfere with each other. As a result, these factors make the SNR of while-drilling seismic data too low and result in the complexity of wave field, in the meantime, make the data processing much more difficult. By using high-order staggered grid FD, it could simulate while-drilling seismic wave field with the different shotpoint depth and different offset distance from the first-order velocity-stress wave equations. Finally, the study on the spatial propagation regulation and characteristics of while-drilling seismic field is able to guide the processing and interpretation of practical while-drilling seismic data.
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  Health Assessment Method of Tank Fire Control System Based on Entropy Weight Method

  LI Yong;CHANG Tianqing;ZHANG Lei;YANG Guozhen

  . 2012, 30(8): 51-54. https://doi.org/10.3981/j.issn.1000-7857.2012.08.007

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  In order to solve the problems exiting in the condition assessment of tank fire control system, such as bigger subjectivity and less accuracy, the health assessment of fire control system based on entropy weight method is studied. Health index model is established on the basis of index system of health assessment. According to the index attributes, the indexes with different dimensions are normalized. The weight of each index is computed by using entropy weight method. Finally, the method is used to assess the health condition of tank fire control system. The assessment results show that stability indexes are the important one that has influence on the general performance of fire control system. It is also proved that the assessment result is reliable and is able to provide some guidelines for the usage and maintenance of fire control system.
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  Plow Plane Multi-level Fuzzy Evaluation Based on Gray Level Correlation Decision Model and Entropy Value Law

  ZHANG Qiang;;SONG Qiushuang;YUAN Zhi;SONG Zhenduo

  . 2012, 30(8): 55-60. https://doi.org/10.3981/j.issn.1000-7857.2012.08.008

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  The evaluation of the thin coal mining plow equipment based on the traditional method suffers from its subjective nature and measurement difficulties. This paper proposes an assessment method of the plow based on the entropy value law and the gray level correlation theory, and the recursive operation is used according to the normalized formula, to calculate membership functions of all levels separately, to establish the gray level correlation decision-making model for the plow. The results show that the evaluation method is feasible, and the plow planning predicted results agree very well with the actual test results. Taking Shaanxi province Nanliang mine as an example, a three-level evaluation model is built for Shanxi province Nanliang mine, for the multi-level fuzzy calculation. The calculation results show that the plow adaptive multi-level fuzzy evaluation analysis system is effective for China coal seam.
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  Design and Implementation of Seed Spread Algorithm for Calculations of Source Flood Submerge Area Based on DEM

  ZHAO Xiuying;WANG Yaoqiang;LI Hongyu;ZHANG Donghua

  . 2012, 30(8): 61-64. https://doi.org/10.3981/j.issn.1000-7857.2012.08.009

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  The recursion and iterative algorithms of source flood submerge area would take too much computer resource, and when the recursion depth is deep, the system would often collapse because of the system stack overflow. To deal with this problem, this paper proposes a source flood submerge model and the related algorithm based on DEM, on the condition of the given water level of the flood. A "seed spreading" algorithm for visiting every node of the stack is developed. First of all, a new empty submerged buffer stack is created, and from the flood source point, it is determined whether the source point's flood level is lower than the given water level. If it is true, the source point is put into the buffer stack and is marked as the submerged grid. Then the surrounding 8 neighborhood raster grids are searched, if a grid meets the condition, we put the grid into the flooded area buffer stack. At the same time, the first element of the stack is popped up. Repeat this operation until the flood buffer stack is empty, and extend this operation to all nodes of the stack, just like the "seed spreading" and eventually complete the inundated area calculation. Compared with the original recursion algorithm, this algorithm has improved the calculation efficiency and stability to a some extent. This algorithm was successfully applied in the "Nanchang flood submerge analysis system".
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  Isolation and Identification of a UDMH Degrading Strain and Its UDMH Degrading Characteristics

  XIA Benli;FAN Chunhua;WANG Xuanjun

  . 2012, 30(8): 65-68. https://doi.org/10.3981/j.issn.1000-7857.2012.08.010

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  Unsymmetrical dimethylhydrazine is a high toxic substance, and the UDMH wastewater has always been an issue. A strain of bacteria was isolated to be used to degrade the UDMH wastewater. The strain of bacteria was screened from the activated sludge, that had been acclimated with the UDMH, and it could grow well in the medium with the UDMH as the only carbon and nitrogen source. It was identified to be Stenotrophomonas by the Biolog microbial identification system after the purification. Its logarithmic phase was from the 12th to the 18th hour in broth. The influencing factors include the temperature, the pH value, the initial UDMH concentration and the added different carbon sources, and their effects on the degradation were investigated. The results show that the optimal temperature and pH value for the strain growth were 30 to 35℃ and 7.2 to 8.0, respectively, and the optimal initial concentration of the UDMH was 50 to 80mg/L. And the addition of glucose could promote the degradation of the UDMH. Under the temperature of 35℃, the medium pH value of 7.2, the initial UDMH concentration of 80mg/L, and the inoculation of 1%, the highest degradation rate of the UDMH was 96.19% in 72h. When the initial UDMH concentration was changed to 120mg/L, the degradation rate of the UDMH was 71.87%, which shows that the strain of bacteria has a certain tolerance to the UDMH.
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  Species Diversity on Moss Soil Microhabitat in Heilongjiang Province

  LIU Peng;TANG Kaixi;CHEN Hui;LIANG Jin;CAI Xinglong

  . 2012, 30(8): 69-72. https://doi.org/10.3981/j.issn.1000-7857.2012.08.011

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  Moss provides ideal biological indicators for global changes, environmental pollution, nutrient conditions, forest integrity and ecosystem health because it is very sensitive to the concentration of atmosphere components and the global climate change. In order to know various species of soil animals and their number in a moss microhabitat, this study collected 400 moss specimens from Maoershan Mountain, Weihushan Mountain, Qixinghe Conservation and Tahe Town in Heilongjiang Province and compared the related data between mountain and wetland habitats. There are a total of 2932 soil animals identified through simple cultivating and they belong to 17 categories. It is indicated that the medium and small soil animals constitute the main part of soil animals in this area, including mites (56.17%) and Collembola (28.39%). It may be speculated that these two types of animals might adapt to the cold environment of the high-latitude with high tolerability and suitability. Tahe Town and Maoershan Mountain have high species diversity and similarity, while Weihushan Mountain and Qixinghe Conservation have low species diversity but high similarity. So, species diversity on the moss soil microhabitat has a remarkable positive correlation with the complexity of the ecosystem and some species can be selected as the standard indicators of the state of the environment. Further studies on response and adaptation of soil animals in the moss to the global changes are needed in the future.
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  Principle and Progress in Balanced Section Technique

  FANG Shi;SUN Qiushi;XIE Rongxiang;HUANG Long

  . 2012, 30(8): 73-79. https://doi.org/10.3981/j.issn.1000-7857.2012.08.012

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  The balanced section technique is a simulation technique about restoring deformation structure into original undeformation condition on the vertical structure, based on geometric conservation principle. It can quantitatively and semiquantitatively analyze the formation tectonic evolution, and access to a wide range of applications in the field of oil-gas exploration and basin modeling. The developing process, basic concept, principle and limiting conditions in simulating process of balanced section technique were introduced. The correcting methods of balanced section were discussed. The making methods of forward modeling and inverse modeling were emphatically elaborated. The insufficiency of the balanced section technique were presented: (1) The accuracy of shortening amount and shortening rate measured by the balanced section technique needs to be improved; (2) The production process of three-dimensional simulation is relatively complicated; (3) The spreading regularity of formation lithology sedimentary and facies should be taken into account; (4) The accuracy of the balanced section technique is largely influenced by the precision of the formation thickness. With deeper study on balanced section technique and the development of the computer technology, the balanced section technique is regarded as an effective method in particular area with violent structural deformation and poor seismic quality.