EKIM Mahsum;ROZI Nurjan;YUSUP Abdiryim
The effect of Uighur medicine Hyssopus officinalis L. ethly aectate on pathological changes in lung tissues of asthmatic rats was observed, and the level of ET-1, the total number of cells and the percentage of lymphocytes (Ly), eosinophils (Eos), and neutrophils (Neu) in BALF, the levels of ET-1, IL-1, IL-6, and TNF-α in serum were determined for the study of the mechanism of its treatment of asthma. The rats were randomly divided into the normal control group, the asthma model group, the aminophylline treatment group, and the Hyssopus ethyl acetate of high, medium and low dose treatment groups. They were sensitized with OVA, Al(OH)3 and DPT vaccine and were then challenged with inhalation of aerosolized OVA solution for preparation of the asthma model. The levels of serum and BALF ET-1 were determined with ELISA, and the total number of cells and the percentages of Ly, Eos, and Neu in BALF and the levels of serum IL-1, IL-6, and TNF-α were determined with radioimmunoassay. The results show that as compared with the asthma model group, the level of ET-1, the total number of cells and the percentage of Ly, Eos, and Neu in BALF, and the levels of serum ET-1, IL-1, IL-6, and TNF-α are significantly lower in those of treated groups(P<0.05). In the comparisons among different dose treatment groups, the level of ET-1, the total number of cells and the percentage of Ly, Eos, and Neu in BALF and the levels of serum ET-1, IL-1, IL-6, and TNF-α are significantly different(P<0.05) and it is shown that the dose-dependent trend to aggravate the inflammatory response of asthma, Hyssopus officinalis L. may inhibit the secretion of ET-1, IL-2, IL-6, TNF-α, Ly, Eos, and Neu, reducing the inflammatory response of asthma.