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   Science & Technology Review
2011, Vol.29, No. 15
28 May 2011

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Science & Technology Review. 2011, 29 (15): 3-3. ;  doi:
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Science & Technology Review. 2011, 29 (15): 10-10. ;  doi:
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Science & Technology Review. 2011, 29 (15): 11-11. ;  doi:
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Science & Technology Review. 2011, 29 (15): 15-27. ;  doi:
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Molecular Sequencing of MODY1-6 Genes in Uighur Early-onset Diabetes Pedigree

MOHEMAITI Patamu;NULI Rebiya;YIMAMU Yilihamujiang;TAXITIEMUER Aierken
Science & Technology Review. 2011, 29 (15): 28-34. ;  doi: 10.3981/j.issn.100-7857.2011.15.001
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Abstract ( 1181 )
Maturity-Onset Diabetes of the Young (MODY) is a monogenic form of diabetes mellitus characterized by autosomal dominant inheritance, early age of onset, and pancreatic beta cell dysfunction. Six MODY genes represent an excellent candidate gene set for identification genetic variation in the MODY family. There is no prior study on the molecular genetics of early-onset type II diabetes in Xinjiang Uighur Autonomous Region and also there is no report on mutations in MODY genes in Uighur ethnic population. The screening for mutations and polymorphism in six known MODY genes (HNF4α, GCK, HNF1α, IPF1, HNF1β, and NEUROD1) in a Uighur family with clinically diagnosed with MODY are studied. One Uighur MODY family from Kashgar City of Xinjiang was involved in this research with its informed consents. Proband and his father were screened for mutations and polymorphism in exon and exon-intron boundaries of six known MODY genes by PCR method followed by direct DNA sequencing. All sequences were analyzed and compared with the reference sequence from NCBI with the Lasergene software (DNASTAR). Seventeen sequence variations are identified and none of them are classified as the pathogenic mutation. Sequence variants of HNF1α gene are relatively more common. HNF1α Exon7 p.Gln497Gln, and NEUROD1 Exon1 c.164G>A are novel variations. Others are all previously described common polymorphisms. No pathogenic mutations or polymorphisms found in GCK. In summary, it is the first report in which six known MODY genes are screened for mutations in Uighur ethnic group. Mutations in MODY1-6 genes might not be the cause of this MODY family. The negative results in MODY genes are provided a further support for the high heterogeneity of this disease. This is the first step for the researches on the MODY genes in Uighur MODY family. The variations which are identified in the study might indicate a relatively high susceptibility to MODY or Type II DM for the Uighur population.

Inhibition of LPS Induced Oxidative Stress by VitC-phosphatide Complex in Mice Peritoneal Macrophage

QI Ce;JIN Qingzhe;WANG Xingguo
Science & Technology Review. 2011, 29 (15): 35-38. ;  doi: 10.3981/j.issn.1000-7857.2011.15.002
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Abstract ( 1150 )
This study makes a comparative analysis of the in vitro anti-oxidative effect of vitamin C(VitC) and VitC phosphate complex (VitC-PC). Mice peritoneal macrophages were prepared and incubated in vitro. Lipopolysaccharide (LPS) of Salmonella was used to induce the oxidative stress. The cells were treated by VitC and VitC-PC of different concentrations, respectively. Extracellular nitric oxide (NO), lactic dehydrogenase (LDH) and malonaldehyde (MDA) and intraocular inducible nitric oxide synthetase (iNOS) were determined to characterize the extent of inflammatory response, the membrane integrity and the lipid peroxidation, respectively. Untreated macrophages were also incubated with VitC or VitC-PC of different concentrations, and the intraocular VitC was measured to estimate the incorporation efficiency of VitC. The results show that the macrophage uptake of VitC-PC is more significant than that of VitC in a high concentration (P<0.05). VitC-PC would inhibit LPS induced macrophage release of NO, and the lipid peroxidation occurs (to generate MDA) and the cell membrane damages (to release LDH), which is more significant than VitC (P<0.05). It is concluded that it is easier for VitC-PC than for VitC to enter into cells and to prevent the damage of high molecules by over production of free radicals.

Sandbody Types and Distribution Characteristics of Triassic System in Akekule Area

LU Jinbo;WANG Yingmin;WU Jiapeng;
Science & Technology Review. 2011, 29 (15): 39-45. ;  doi: 10.3981/j.issn.1000-7857.2011.15.003
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Abstract ( 424 )
Based on the analysis of core, logging, and seismic data, the sedimentation characteristics are studied based on the systems tracts stratigraphic framework of Triassic system in Akekule Area. Three sedimentary facies including braided river delta, lake, sublacustrine fan, and seven subfacies are recognized. There are seven kinds of sand bodies; they are distributary channel sands, chief subaqueous distributary channel sands, subordinate subaqueous distributary channel sands, mouth bar sands, feeder channel sands, braided channel sands, and distal sheet sands. The distributions of sand bodies in different periods are identified, and three models for the distribution of sand bodies, which are braided river delta of shallow gentle slope, braided river delta with slope break zone-sublacustrine fan, and normal braided river delta, are established. The paleogeomorphology plays an important role in the distribution of sand bodies.

Fractures Characteristics of Conglomerate Reservoirs and Their Influence on Development Effectiveness: A Case Study of the Urho Reservoir of NO. 8 District of Karamay Oil Field

YIN Guofeng;XU Huaimin;ZHANG Guangqun;TAO Wulong;ZHANG Bing
Science & Technology Review. 2011, 29 (15): 46-51. ;  doi: 10.3981/j.issn.1000-7857.2011.15.004
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Abstract ( 462 )
The Lower Urho reservoir of NO. 8 district of Karamay oil-field is characterized by complicated fracture distribution, low porosity, and strong heterogeneity, etc. In view of these characteristics, by using core, well log, dynamic production data, these contents are analyzed, which are the fracture characteristics, main control factors of fracture formation, and influence on development effectiveness, etc. Development adjustment proposal measures of fracture conglomerate reservoir were put forward based on the fracture research. The concrete steps are as follows: Firstly, the types and characteristics of fracture conglomerate reservoir are defined on the basis of reservoir cores analysis and imaging logging data interpretation. Secondly, the main control factors of fracture formation is revealed, the major fracture strike is determined, and the fracture distribution and plane extension in the oil reservoir are summarized by analyzing the relation among regional tectonic evolution, the change of stress fields, and the types of sedimentary and fracture formation. Finally, by combining dynamic production data of injection-production well group, the influence of fracture identification on development effectiveness is analyzed. And technology policies on fractured conglomerate reservoir development are pointed out; the policies effectively apply the fracture research results on the adjustment of injection production well pattern, the adjustment of well design, and selection of injection parameters. The practical results show that both the deliverability of oil well and water flooding, water breakthrough, and high water content in well are controlled by fractures development degree and distribution pattern. And a good spatial disposition relationship between injection production wells and fracture is about 45°. High efficiency development for the low-permeability fractured conglomerate reservoir is obtained by the close spacing development, whose characteristics are low water injection rate, five points well pattern form of law, and linear injection. And they could be certainly applied to other similar reservoirs.

Screening Bioemulsifier-produced Microorganisms and Its Function Analysis

XU Shuang;HUANG Zhiyong;LU Fuping;WANG Yongli
Science & Technology Review. 2011, 29 (15): 52-57. ;  doi: 10.3981/j.issn.1000-7857.2011.15.005
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Abstract ( 405 )
Three thermophilic bacteria were isolated from the crude oil contaminated soil samples gathered from Yaerxia oil reservoirs of Yumen oilfield in Gansu Province. According to their 16S rRNA gene analyses, and the physiological properties, the isolated XS1 was identified as Brevibacillus sp; and the isolated XS2 and XS3 were identified as Geobacillus pallidus. The three isolates could degrade crude oil and had effective emulsification activity. All the isolates can grow at a high temperature, with the optimal growth temperature of XS2 being 60℃, and have a wide adapt-ability to pH, in the range of 5.5—9.5. NaCl concentration has no obvious effect on the growth of three strains. Meanwhile, XS2 has an effective emulsification activity; and the emulsification activity has no apparent change at 100℃ and under 20% salinity conditions. The analysis of chromatogram characteristic changes of crude oil during biodegradation process shows that the strain XS2 can degrade not only the light-weight components of crude oil, but also its heavy-weight components, it enhances the fluidity of crude oil, and the quality of crude oil is also improved. It will have a good prospect in microbial enhanced oil recovery.

Prediction of Aqueous Solubility and Partition Coefficients of Halogenated Benzenes Using Molecular Fragments Variable Connectivity Index

CHEN Qiang;SUN Jingmin;HU Liang
Science & Technology Review. 2011, 29 (15): 58-61. ;  doi: 10.3981/j.issn.1000-7857.2011.15.006
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Abstract ( 420 )
For the molecular fragments Variable Connectivity Index (mfVCI), the molecular fragments are defined as the atoms or functional groups and are also regarded as the molecular structure unit for the major influence of the property. Different molecular fragments were endued with different fragments weights. In order to test whether or not the mfVCI could be used for predicting different properties of the same molecules, the Quantitative Structure-Property Relationship (QSPR) models of aqueous solubility (Sw) and n-octanol/water partition coefficients(Kow) for 36 halogenated benzenes based on the mfVCI were investigated. The fitting goodness of the QSPR models are mainly characterized by determination coefficient (R2), standard deviation (SD), Fischer variance ratio(F), and significant lever (P). The internal cross-validation including leave-one-out and leave-many-out methods with cross-validated correlation coefficients (qloo2 and qlmo2) was used to evaluate the model's robustness. The external cross-validation was used to evaluate the predictive power of the models developed from the training set by correlation coefficients (qext2). The linear relationship was assumed between mfVCI and Sw or Kow in here. The mfVCI was optimized by using Solver. The target function is the root mean calculated residual sum of squares for training set. The QSPR models with R2 value of more than 0.97, q2 value of more than 0.89, and qext2 value of more than 0.95 have the good robustness and predictive ability as well as fitting ability. The mfVCI could distinguish the different role of same atoms or functional groups among different chemical bonding. The results show that the mfVCI could be well used in the prediction of both of aqueous solubility and n-octanol/water partition coefficients for halogenated benzenes. It could describe different properties equally well. The mfVCI maybe play an important role in the development of molecular descriptors on QSPR researches.

An Algorithm of Rapidly Determining Grid-fault Voltage Based on the Principle of Compensation in Current and Voltage

LIU Guangye;CHENG Shan;WANG Yang
Science & Technology Review. 2011, 29 (15): 62-65. ;  doi: 10.3981/j.issn.1000-7857.2011.15.007
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Abstract ( 386 )
This paper proposes an algorithm of rapidly determining the node voltage based on current and voltage compensation. The pre-contingency voltage is calculated by Newton power flow method, and the derivatives of first 2 orders of current and voltage with respect to branch outage parameters are calculated by using the convergent power flow equation of the base network topology. The values of current and voltage are obtained by using the Taylor series expansion. Lines out of operation(excluding the generator) based on the power injection remain unchanged, the node's current and voltage satisfy the compensation principle, the function of the node voltage with respect to the fault branch parameters is obtained by calculating the average compensation of current and voltage as the voltage Taylor expansion of the correction. The advantage of this method 1ies in the fact that the admittance matrix is not to be re-factorized after one branch is out of operation, and the accurate node voltage can be obtained by using only a low order Taylor expansion, to meet the requirements of calculation speed and accuracy. The feasibility and the efficiency of the proposed method are tested on the standard IEEE system.

Hydrogeological Features and Measures for Water Control in Dingfengshan Mine

XU Fumei;LEI Fangfang;WU Zhijie;WU Chaofan
Science & Technology Review. 2011, 29 (15): 66-69. ;  doi: 10.3981/j.issn.1000-7857.2011.15.008
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Abstract ( 455 )
The groundwater system in Dingfengshan Mine includes quaternary pore aquifer group, fissure aquifer group of coal bed rock, fissure-karst aquifer group of Qixia limestone, and goaf water; the water in the system is generated from atmospheric precipitation, aquifer group, faulted structural belt, and goaf water, among them the faulted structural belt and goaf water are the major risks for the mine water inrush. The hydraulic conductivity of faulted structural belt, which is originated with in the mine, allows karst water to go into the coal mine, the key tasks for the water control in the mine focus on the investigation of the faulted structural belt and goaf water area. Methods for probing water conductivity belt mainly could rely on the combination of high-resolution three-dimensional seismic exploration with transient electromagnetic method for detecting; while methods of water prevention, according to the actual situation of maximum security, could be based on stay watertight coal (rock) column closure water shutoff, hydrophobic buck, and grouting reinforcement measures. As the goaf water is mainly distributed in north fault F5, the method for probing it is the combination of drilling with geophysical exploration, and methods for prevention are mainly building coal and rock pillars of waterproofing, probing water regularly, and leading it away in time.

Progress of Iron Ore Sintering Technology

JIANG Xi;PEI Yuandong;HAN Hongliang
Science & Technology Review. 2011, 29 (15): 70-74. ;  doi: 10.3981/j.issn.1000-7857.2011.15.009
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Abstract ( 1251 )
The quality of the sinter influences its behavior in the blast furnace and the technical parameters of the blast furnace. The quality and the properties of the sinter are mainly determined by the properties of the iron ore powder. In recent years, the iron and steel industry faces with increasing cost pressures, as the quality of the powder iron ore deteriorates and its price increases. Meanwhile, an important progress is made in the technology of the iron ore sintering, especially in the development of the low temperature sinter and high-depth sinter, the improvement of limonite technology, the design of pre-reduction sintering and Mosaic Embedding Iron Ore Sintering (MEBIOS), and the improvement of high temperature properties of iron ore sintering, which will be briefly reviewed in the present paper. These technologies are aimed for more efficient use of the iron ore powder resource, related with concerns for resource degradation and environmental deterioration. The quality of ore is only a relative concept - a "low" quality ore can become relatively "high" if its strengths are exploited and weaknesses suppressed. These new technologies can reduce the consumption of ore and improve the quality of the final product, which are characteristic of a "good" ore, which means the efficient use of inferior resources.

Development of Hydrophobically Associating Polyelectrolyte

AN Huiyong;CHEN Qiang;SONG Chunlei
Science & Technology Review. 2011, 29 (15): 75-79. ;  doi: 10.3981/j.issn.1000-7857.2011.15.010
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Hydrophobically Associating Water-soluble Polymers (HAWPs) had potential applications in many industrial societies due to their special properties. Recently Hydrophobically Associating Poly-Electrolytes (HAPEs) had attracted increasing interests because they possessed high water-solubility and peculiar solution property and realized multi-responsive properties, which all stemmed from their ionic groups. In this paper, HAPEs are classified into Type I (ionic units and hydrophobic groups from different monomers) and Type II (ionic units and hydrophobic groups from the same monomer) according to the relatived position between ionic units and hydrophobic groups and present research state of various kinds of HAPEs, such as anionic HAPEs, cationic HAPEs and amphoteric HAPEs, was reviewed according to the different types of ions. Suggestions and development trends of the research field are put forward in terms of the present situation and betaine HAPEs will become a hot spot of this research field.

Science & Technology Review. 2011, 29 (15): 80-80. ;  doi:
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