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   Science & Technology Review
2009, Vol.27, No. 0904
28 February 2009

Articles
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Tanshinone Effect on theExpression of IRS-1 and p-ERK in Trophoblastic Cells Under Insulin Resistance

Science & Technology Review. 2009, 27 (0904): 75-79. ;  doi:
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Abstract ( 503 )
This paper investigates the expression of mitogen-
activated protein kinase and insulin receptor substrate-1 in
trophoblastic cells and the relationship with hypertensive disorder complicating pregnancy. With primary culture of human placental villus in vitro, trophoblastic cells were exposed to 2.5 ?滋mol/L wortmannin for 48 hours to establish insulin-resistant trophoblastic cell. Protein expression of insulin receptor substrate-1 and extracellular signal-regulated kinase was obtained by western
blotting. Under the microscope, the polygon cell took the shape of monolayer slice and there were some syncytio-trophoblasts by cell fusion; with insulin resistance, the level of insular signal transmission of the main protein IRS-1 was risen 26.58% above the normal level. With treatment of tanshinone, it was reduced 16.42% as compared with the model. With treatment of metformin, the level of insular signal transmission of the main protein IRS-1 reduced 6.66% as compared with the model. With insulin resistance, protein expression of extracellular signal-regulated kinase was risen 56.93% above the normal level. With treatment of tanshinone, protein expression of extracellular signal-regulated kinase reduced 26.63% as compared with the model. With treatment of metformin, protein expression of extracellular signal-regulated kinase reduced 10.18% as compared with the model. It is concluded that with the trophoblastic cell in insulin resistance, the protein expression of IRS-1 and p-ERK will rise. Use of tanshinone2 and metformin will reduce the protein expression of IRS-1 and p-ERK.

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