. 2009, 27(0904): 75-79.
This paper investigates the expression of mitogen-
activated protein kinase and insulin receptor substrate-1 in
trophoblastic cells and the relationship with hypertensive disorder complicating pregnancy. With primary culture of human placental villus in vitro, trophoblastic cells were exposed to 2.5 ?滋mol/L wortmannin for 48 hours to establish insulin-resistant trophoblastic cell. Protein expression of insulin receptor substrate-1 and extracellular signal-regulated kinase was obtained by western
blotting. Under the microscope, the polygon cell took the shape of monolayer slice and there were some syncytio-trophoblasts by cell fusion; with insulin resistance, the level of insular signal transmission of the main protein IRS-1 was risen 26.58% above the normal level. With treatment of tanshinone, it was reduced 16.42% as compared with the model. With treatment of metformin, the level of insular signal transmission of the main protein IRS-1 reduced 6.66% as compared with the model. With insulin resistance, protein expression of extracellular signal-regulated kinase was risen 56.93% above the normal level. With treatment of tanshinone, protein expression of extracellular signal-regulated kinase reduced 26.63% as compared with the model. With treatment of metformin, protein expression of extracellular signal-regulated kinase reduced 10.18% as compared with the model. It is concluded that with the trophoblastic cell in insulin resistance, the protein expression of IRS-1 and p-ERK will rise. Use of tanshinone2 and metformin will reduce the protein expression of IRS-1 and p-ERK.
