08 March 2014, Volume 32 Issue 7

    

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Articles
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  Recombinant Vascular Endothelial Receptor F3 Peptide-PE39KDEL Protein can Inhibit Tumor Growth

  GUO Kai, WANG Yilian, ZHOU Hongzi, CHEN Quan, CHEN Kai, LI Jishun, HU Jindong, YANG Hetong

  journal1. 2014, 32(7): 15-21. https://doi.org/10.3981/j.issn.1000-7857.2014.07.001

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  The objective was to investigate the antitumor effect of F3-PE39KDEL in vitro and in vivo. MTT method was employed to determine the inhibiting effect of F3-PE39KDEL on MCF-7, A549, SKOV3 and HepG2 cell lines. Its toxicity in mice was observed using the median lethal dose method. Its antitumor effect was determined based on the transplanted A549 cell line in nude mice. The results showed that when the culture times were 72 h and 120 h, the F3-PE39KDEL showed significant inhibitory effect on A549 and MCF-7 cell lines, with IC₅₀ values of 0.41 and 0.42 μg/mL, and 4.95 and 2.53 μg/mL, respectively. The toxicity test showed that the median lethal dose was 1.1782 mg/kg. The animals' spontaneous movement was decreased after administration, and animals died mainly within 1d after administration. All the animals including the dead and alive showed no abnormalities after sectional examination. The F3-PE39KDEL showed a potential antitumor effect in vivo. At the doses of 0.1, 0.2 and 0.4 mg/kg, its inhibitory rates were 37.0%, 43.2% and 53.1%, respectively, and the dose-activity relationship was also observed. These results suggest that the F3-PE39KDEL has good prospects in terms of targeted cancer therapy.
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  Effects of α-Synuclein Protein Overexpression on Mice Striatum

  DUAN Jinyan, LIU Kefu, ZHANG Yongqian, ZHANG Yanyan, QING Hong, DENG Yulin

  journal1. 2014, 32(7): 22-26. https://doi.org/10.3981/j.issn.1000-7857.2014.07.002

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  Parkinson's disease (PD) is a common progressive neurodegenerative syndrome. The α- synuclein(α- syn)is a major protein constituent of Lewy bodies, and the α-syn A30P mutant causes familial autosomal dominant PD. In the present study, we explored the effect of α-syn overexpression and its mutant α-syn A30P on mice striatum. The behavior of PDGF-h α-synuclein^WT and PDGF-h α-synuclein^A30P transgenic mice was investigated, and the oxidative stress levels and the concentration of catecholamine isoquinolines (CAIQs) were determined in transgenic mice striatum, respectively. Compared to the control group, the coordination abilities of the two types of transgenic mice decreased. In addition, the level of oxidative stress and the concentration of CAIQs significantly increased in the overexpression of α-syn WT and α-syn A30P mice striatum. Thus, it is concluded that overexpression of α-syn and its mutant α-syn A30P exhibit impairment in the striatum through increasing the level of oxidative stress and CAIQs.
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  Improvemental Effects of Arbuscular Mycorrhizal Fungi and Humic Acid on Sandy Soil in Shendong Mining Area

  WANG Yi, YANG Yan, LI Shaopeng, LIU Rongrong, BI Yinli

  journal1. 2014, 32(7): 27-32. https://doi.org/10.3981/j.issn.1000-7857.2014.07.003

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  Mining activities have severe deleterious effects on plants and soil fertility that must be ameliorated with additional amendments in order to achieve successful ecological restoration in the Shendong mining area in Shaanxi Province. Pot experiment is set up to investigate combined effects of arbuscular mycorrhizal fungi and humic acid on the mining soil. The results show that the combined effect can improve the maize biomass and root vigor. When humic acid whose concentration is 0.05% is added, the dry weight of maize is 73% higher than the controled, and the available phosphorus content and acid phosphatase activity are improved by 30% and 49%, respectively, indicating that the improvement effect on maize growth and soil is optimal. The hyphal density in rhizosphere soil of maiz is 0.8 m/g higher than that of the controled, and microorganisms in rhizosphere soil is increased significantly. Combined effects of arbuscular mycorrhizal fungi and humic acid are conductive to maize growth and the degraded soil improvement.
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  Research on the Evolution of Subsequent Yield Surfaces in Stress Space and Strain Space

  FU Qiang, LIU Fang, CHEN Cen

  journal1. 2014, 32(7): 33-38. https://doi.org/10.3981/j.issn.1000-7857.2014.07.004

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  The shape and position of yield surface have a direct influence on the determination of plastic deformation of materials. Considering that slip is the main plastic deformation mechanism, the extension of single crystal plasticity called slip-component model is introduced, and the evolution of subsequent yield surfaces in stress space and strain space is investigated. The method to determine the yield surfaces in stress space and strain space is proposed. A combined kinematic-distortional hardening model is developed to describe the translation and distortion of subsequent yield surfaces in stress space and strain space. Numerical simulations of the evolution of subsequent yield surfaces in (σ₁₁ -σ₁₂) stress space and (ε₁₁ -γ₁₂) strain space are performed under pure torsion and combined tension- torsion loading for aluminum 1100- O. The results show that the agreement between the predictions and experiments is quite satisfactory. The work demonstrated that whether in stress space or strain space, based on the latent hardening and Bauschinger effect of the slip component, the subsequent yield surface can be described that the forward part inflates and the rear part deflates so that the subsequent yield surface has a sharp front and a blunt rear.
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  Splitting Stress Analysis of Rock with Rectangular Shape

  WANG Zhi, ZHANG Jingfei, DU Yunhai

  journal1. 2014, 32(7): 39-43. https://doi.org/10.3981/j.issn.1000-7857.2014.07.005

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  The stress distribution of rectangular rock under split loads is an important research topic of rock mechanics. The formula of rectangular specimen tensile stress under splitting loads can be deduced by the Fourier series solution of the elastic plane problem. To compare the theoretical solution and the finite element solution, a dimensional finite element model of the rectangular specimen is built under the same loading conditions. The stress distribution and the impact of the width of spacer and the width to length ratio are analyzed. It is shown that results of the theoretical solution and the finite element solution are consistent. The maximum tensile stress does not appear in the center of the specimen but in a certain point with coordinates. The maximum tensile stress decreases with the increasing of the width of spacer and the width to length ratio b/a. The tensile stress distribution patterns of cylindrical specimen and square specimen are consistent, but the tensile stress of the square specimen is greater than that of the cylindrical specimen. It is proved that the splitting stress distribution of rectangular rock obtained by the Fourier series analysis method is consistent with the rock-like material splitting experimental results.
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  Study and Design of Temperature Wireless Monitoring System for Heavy Commercial Vehicle Brake Drum

  ZHAO Xuan, LIU Qiang, LIU Rui, LUO Qingdong, YU Qiang

  journal1. 2014, 32(7): 44-50. https://doi.org/10.3981/j.issn.1000-7857.2014.07.006

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  Aimming at the safety problem of heavy commercial vehicles running on long downgrades, a temperature wireless monitoring system for brake drum based on NewMsg_RF2401 is presented in this paper. An overall design program of the temperature monitoring system for brake drum is given as well. The hardware and software including the minimum system circuit of Freescale S12 MCU, the signal acquisition module, the wireless transceiver module and the MCGS human-computer interaction intelligent instrument are designed and implemented. Finally, the loading road experiment for the monitoring system is provided. The results prove that the temperature monitoring system for heavy commercial vehicle brake drum could monitor the temperature in real time, achieve intelligentization, visualization, security and stability, and improve the safety of running on long downgrades for heavy commercial vehicles.
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  Staged Well Killing Mathematical Model and Simulation for Bullheading

  SUN Xiaofeng, YAN Tie, WANG Kelin, WU Yanze, ZHANG Yang

  journal1. 2014, 32(7): 51-55. https://doi.org/10.3981/j.issn.1000-7857.2014.07.007

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  A bullheading process could be divided into three stages based on the gas compressibility law and the gas-liquid twophase flow law, and a mathematical model for each stage with consideration of the gas slippage is developed to quantitatively calculate the bottom pressure and the casing pressure under different well killing conditions. The simulations of the bullheading parameters show that, the kill time decreases and the bottom pressure and the casing pressure increase with the increase of the pump displacement; both the formation permeability and the permeable formation height have a little influence on the pressure in the first stage, but when the high permeable formation or the high height formation enters into the second and third stages, both the bottom pressure and the casing pressure decrease with the increase of the formation permeability or the formation height, and the change range becomes small. In addition, typical bullheading curves are obtained by analyzing the characteristics of the bottom pressure and the casing. The predicted model had a good agreement with the field test. This can provide a theoretical basis and guidance for the selection of killing methods and the design of bullheading.
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  Influencing Factors on Deformation of Blade Rolling Process

  MAO Jun, CAO Zhi, DONG Xiaotong

  journal1. 2014, 32(7): 56-61. https://doi.org/10.3981/j.issn.1000-7857.2014.07.008

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  For a certain grade of the aviation engine stator blade surface machining, complex shapes may cause a difficult situation of blade deformation control. Through the high-order curve fitting method, the three-dimensional model of blank moleds and blades are established, and ANSYS/LS-DYNA software is used to simulate the blade rolling process. According to the results of the simulation of the blade cross section, nodes are select to study the blade surface's normal and lateral deformations caused by the rolling friction coefficient, the rolling speed, the rolling amount of pressure. Different parameters influencing the deformation of blade surface and the laws of the rolling process are obtained. The results show that the maximum deformation area of the blade is in the dorsal arc position of the steep slope, where the blade surface curvature is large. The error is also generated by the maximum strain. The deformation amount of the blade increases with the rolling friction coefficient decreasing, reduces with the rolling speed increasing, and increases with the rolling amount of pressure increasing. The results can provide a theoretical reference for a certain grade aircraft engine stator blade processing design.
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  Analysis of Rupture Failure of Sidetrack Deep Well Pipe in Tahe Oilfield

  ZHANG Zhihong, ZHANG Jiangjiang, GAO Qiuying, SUN Haijiao, XIAO Wenwen

  journal1. 2014, 32(7): 62-66. https://doi.org/10.3981/j.issn.1000-7857.2014.07.009

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  In the heavy oil zone with high content of CO₂ and H₂S in Tahe Oilfield, a sidetrack tubing downhole of P110S had a typical fracture failure. In order to find out the fracture reasons, this paper combined the downhole tubing service working conditions to test and analyze the pipeline rupture in terms of chemical composition, mechanical properties, and microstructure morphology, and analyzed the energy spectrum characteristic of the corrosion products in the fracture site. It is recognized that the well tubing corrosion is the main reason of the fault. Through the analysis of two kinds of typical corrosion characteristics of the tubing sidetrack, it is recognized that the corrosion and pitting mechanism of the tubing wall is mainly a typical CO₂ electrochemical corrosion, and that for the side wall of the groove-like distribution of corrosion and pitting, the corrosion mechanism is electrochemical corrosion and crevice corrosion in the condition of the acid with low pH. Targeted prevention measures are also proposed for pipeline corrosion perforation on typical corrosion issues.
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  Basal Respiration Influence Factors of Petroleum Polluted Soil

  WANG Wei, ZHANG Yuling, MA Zhen, CHEN Peng

  journal1. 2014, 32(7): 67-70. https://doi.org/10.3981/j.issn.1000-7857.2014.07.010

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  The petroleum pollutants permeating into the soil environment will affect the soil physical and chemical properties and microbial activity, which in turn affects soil respiration intensity. Some certain pollution area is taken as the research object in this paper, and through surveying and sampling，and by means of molecular biology method, the soil microbial biomass, soil chemical indicators, and soil basal respiration of the polluted vadose and aquifer are studied. It is shown that the basal soil respiration is mainly affected by microorganism quantity, followed by nitrate, ammonia nitrogen, nitrite, etc. The influence of oil pollution on soil basic respiration is mainly the inhibitory effect in the shallow soil depth (0.5 m) and in the underground water level change area (1.5 m).
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  Characteristics of Tight Oil Reservoir and A Novel Key Technique for Its Development

  FANG Wenchao, JIANG Hanqiao, SUN Binfeng, GU Hao, LIU Hongxia

  journal1. 2014, 32(7): 71-76. https://doi.org/10.3981/j.issn.1000-7857.2014.07.011

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  As shown by the actual reservoir development, for ideal tight oil reservoirs, we have two typical kinds of source-reservoir relationship, i.e., the source contacts with the reservoir and the source adjoins the reservoir, and the ideal shale oil is not included in the tight oil . Thus, the definition and the classification of the tight oil are clear. The characteristics of the tight oil reservoirs are summarized, including the aspects of the structure, the petrophysics, and the fluid property. In China, the tight oil has a huge resource potential, which is the most realistic alternative in the unconventional oil and gas development field. HiWAY flow-channel hydraulic fracturing technique has a huge potential in the development of the tight oil, which has been applied in more than forty unconventional oil and gas fields in ten countries successfully, increasing the production by more than 20% while saving plenty of water and proppant. The keys of this new technique are the alternative and intermittent injection of the proppant and the high intensive gelled fracturing fluid, and a patented fabric additive keeping the stable distribution of flow-channel. This technique has not been applied in China so far.
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  Geological Characteristics of Iron Ore Deposits in the Western Tianshan Mountains Metallogenic Belt and Its Research Situation

  ZHANG Xi

  journal1. 2014, 32(7): 77-83. https://doi.org/10.3981/j.issn.1000-7857.2014.07.012

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  Many marine volcanic type iron oxide ore deposits are exposed along the Awulale Iron Metallogenic Belt in the eastern part of the Yili block, which is an elongated wedge-shape tract tapering to a point. Recently, numerous large-scale high-grad iron ore deposits have been found consecutively in the Western Tianshan Orogen with the new round of ore prospecting exploration and evaluation in Xinjiang province, especially in the Awulale Iron Metallogenic Belt. Therefore, this metallogenic belt has become a significant high-grade iron metallogenic belt in Xinjiang province and even in China. However, these iron ore deposits have so far insufficient studies on metallogenesis. Additionally, the metallogenic tectonic setting is still a subject of the intense discussion. Furthermore, the enrichment mechanism of Fe element and the indicator for further prospecting should be summarized urgently. Based on the research progress of iron ore deposits in the world, in this contribution we summarize the geological characteristics and present the research situation of the significant iron ore deposits in the Western Tianshan Mountains Metallogenic Belt. We also discuss the existing problems in the research and propose some research prospects in order to promote the study on the metallogenesis of these ore deposits and further ore prospecting exploration in the Western Tianshan Mountains.