CHEN Yin, ZHONG Meizuo, UPUR Halmurat, MAMTIMIN Batur, DENG Wanli, ZHANG Hongliang, WANG Rui
Metabonomics studies on plasma and urine of patients with breast cancer using NMR spectroscopy are to establish metabolic markers correlated with breast cancer to guide clinic diagnosis and provide the new ideas and methods. In this study, a total of 45 patients with breast cancer were enrolled and 50 healthy volunteers as controls. Meanwhile, blood and urine samples from the subjects were collected by the researchers for testing. For each sample, the 1H nuclear magnetic resonance (NMR) based metabonomic analysis was performed, the subsection integral of the free attenuation signal was computed, and the obtained data were analyzed by the orthogonal partial least-squares discriminant analysis (OPLS-DA). The results of the study show that compared with the healthy people, leucine, valine, phenylalanine, tyrosine, alanine, histidine, and glycine are significantly decreased (P<0.05); lipid (LDL), beta glucose，lactic acid, choline, inositol and creatine are significantly reduced (P<0.05); glycoprotein, malonic acid, acetoacetic acid, beta hydroxybutyric acid, and acetone are increased significantly (P<0.05) in the plasma of patients with breast cancer. Moreover, isoleucine, valine, glutamic acid, histidine, dimethylamine, hippuric acid, betaine, taurine, succinic acid, citric acid,creatine, and creatinine are decreased (P<0.05) and Lactic acid increased (P<0.05) in urine of patients with breast cancer. Through this study, metabolic markers correlated with breast cancer using changes of metabonomics in plasma and urine have been established.
