MAMTIMIN Batur, XU Weifang, LI Wei, WU Yan, UPUR Halmurat
To investigate the plasma samples from asthma patients with phlegm-stasis or abnormal Savda syndrome with NMR spectroscopy and to analyze their metabolic varieties, characteristics and reciprocity, fifty healthy volunteers who were confirmed by medical examination and 162 asthma patients were enrolled. The asthma patients were divided into the abnormal Savda syndrome group and the phlegm-stasis syndrome group according to the theory of traditional Uyghur medicine and the theory of traditional Chinese medicine, respectively. There were 80 cases in the abnormal Savda syndrome group and 82 in the phlegm-stasis group. Plasma samples were obtained by centrifugation of blood samples from all the individuals in the morning before breakfast, and were immediately stored at -80℃ until use for NMR spectroscopy. 1H NMR spectra were analyzed using the orthogonal projection to latent structure with the discriminative analysis (OPLS-DA) method with unit variance scaling. The discriminative significance of metabolites was determined by the Pearson's product-moment correlation coefficient. The results showed that compared to the control group, the asthma patients with phlegm-stasis had low concentrations of isoleucine, leucine, valine, lactate, alanine, glycoprotein, citrate, tyrosine, phenylalanine and 1-methylhistidine (P<0.05); the asthma patients with abnormal Savda syndrome had low concentrations of isoleucine, leucine, valine, lactate, alanine, glycoprotein, glutamine, creatine, creatinine, citrate, tyrosine, phenylalanine and 1-methylhistidine, α-glucose and β-glucose (P<0.05). The conclusion is that the asthma patients with different syndromes have very similar metabolic changes, i.e., abnormal performance for amino acid metabolism, inhibited citric acid cycle gluconeogenesis disorder and immune dysfunction.
