LIU Lihua, PANG Binshuang, LIU Yangna, LI Hongbo, WANG Na, ZHANG Xin, ZHANG Xiaoxu, ZHAO Changping
Correct judgment of contaminated plants in wheat hybrid is the premise for accurate identification of seed purity. In this study, taking the hybrid wheat variety "Jingmai1100" as an example, we propose a method for judging contaminated plants in hybrid wheat using SSR markers on the basis of analysis of 180 wheat hybrids: 1) Not less than five polymorphic SSR markers on parents of different genotypes should be screened; 2) Polymorphic SSR primers are used to detect the genotype of parents and hybrids, and each plan's genotype is recorded according to the recording principle of hybrid genotypes; 3) True hybrid genotypes should be judged accurately, with the following principle: No matter whether there is the homozygous locus in parents, as long as the individual has both parents' genotype, it should be regarded as a true hybrid genotype; 4) Judgment of contaminated plants in hybrids: When the individual is different from true hybrid on multiple SSR loci, it could be regarded as a contaminated plant.
